Browsing by Author "NOLAN, DEREK"
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Apolipoprotein L-I promotes trypanosome lysis by forming pores in lysosomal membranes
NOLAN, DEREK (American Association for the Advancement of Science, 2005)Apolipoprotein L-I is the trypanolytic factor of human serum. Here we show that this protein contains a membrane pore-forming domain functionally similar to that of bacterial colicins, flanked by a membrane-addressing ... -
Characterization of two protein disulphide iosmerases from the endocytic pathway of Trypanosoma brucei
NOLAN, DEREK (American Society for Biochemistry and Molecular Biology, 2005)Proteins from the endocytic pathway in bloodstream forms of Trypanosome brucei are modified by the addition of linear poly-N-acetyllactosamine side chains, which permits their isolation by tomato lectin affinity chromatography. ... -
Coupling of lysosomal and mitochondrial membrane permeabilization in trypanolysis by APOL1.
NOLAN, DEREK (2015)Humans resist infection by the African parasite Trypanosoma brucei owing to the trypanolytic activity of the serum apolipoprotein L1 (APOL1). Following uptake by endocytosis in the parasite, APOL1 forms pores in ... -
A differential role for actin in the life cycle of T. brucei.
NOLAN, DEREK (Nature Publishing Group, 2004)Actin is expressed at similar levels but in different locations in bloodstream and procyclic forms of Trypanosoma brucei. In bloodstream forms actin colocalizes with the highly polarized endocytic pathway, whereas in ... -
Identification and Characterization of a Stage Specific Membrane Protein Involved in Flagellar Attachment in Trypanosoma brucei
NOLAN, DEREK (2013)Flagellar attachment is a visibly striking morphological feature of African trypanosomes but little is known about the requirements for attachment at a molecular level. This study characterizes a previously undescribed ... -
Identification and Characterization of an Unusual Class I Myosin Involved in Vesicle Traffic in Trypanosoma brucei
NOLAN, DEREK; LEDDY, NEAL (2010)Myosins are a multimember family of motor proteins with diverse functions in eukaryotic cells. African trypanosomes possess only two candidate myosins and thus represent a useful system for functional analysis of these ... -
Metabolic reprogramming during the Trypanosoma brucei life cycle
NOLAN, DEREK; Smith, Terry K.; Bringaud, Frédéric; Figueiredo, Luisa M. (2017)Cellular metabolic activity is a highly complex, dynamic, regulated process that is influenced by numerous factors, including extracellular environmental signals, nutrient availability and the physiological and developmental ... -
TrypanoCyc: a community-led biochemical pathways database for Trypanosoma brucei.
NOLAN, DEREK (2015)The metabolic network of a cell represents the catabolic and anabolic reactions that interconvert small molecules (metabolites) through the activity of enzymes, transporters and non-catalyzed chemical reactions. Our ...