dc.contributor.author | O'Neill, Luke | |
dc.date.accessioned | 2024-07-02T08:19:42Z | |
dc.date.available | 2024-07-02T08:19:42Z | |
dc.date.created | 2023 | en |
dc.date.issued | 2023 | |
dc.date.submitted | 2023 | en |
dc.identifier.citation | Wright O, Harris A, Nguyen V.D, Zhou Y, Durand M, Jayyaratnam A, Gormley D, O� Neill L.A.J, Triantafilou K, Nichols E.M, Booty L.M, C5aR2 Regulates STING-Mediated Interferon Beta Production in Human Macrophages, Cells, 12, 23, 2023 | en |
dc.identifier.other | Y | |
dc.identifier.uri | https://hdl.handle.net/2262/108660 | |
dc.description | PUBLISHED | en |
dc.description.abstract | The complement system mediates diverse regulatory immunological functions. C5aR2, an enigmatic receptor for anaphylatoxin C5a, has been shown to modulate PRR-dependent pro-inflammatory cytokine secretion in human macrophages. However, the specific downstream targets and underlying molecular mechanisms are less clear. In this study, CRISPR-Cas9 was used to generate macrophage models lacking C5aR2, which were used to probe the role of C5aR2 in the context of PRR stimulation. cGAS and STING-induced IFN-β secretion was significantly increased in C5aR2 KO THP-1 cells and C5aR2-edited primary human monocyte-derived macrophages, and STING and IRF3 expression were increased, albeit not significantly, in C5aR2 KO cell lines implicating C5aR2 as a regulator of the IFN-β response to cGAS-STING pathway activation. Transcriptomic analysis by RNAseq revealed that nucleic acid sensing and antiviral signalling pathways were significantly up-regulated in C5aR2 KO THP-1 cells. Altogether, these data suggest a link between C5aR2 and nucleic acid sensing in human macrophages. With further characterisation, this relationship may yield therapeutic options in interferon-related pathologies. | en |
dc.language.iso | en | en |
dc.relation.ispartofseries | Cells; | |
dc.relation.ispartofseries | 12; | |
dc.relation.ispartofseries | 23; | |
dc.rights | Y | en |
dc.subject | complement | en |
dc.subject | anaphylatoxin C5a | en |
dc.subject | regulatory immunological functions | en |
dc.subject | interferon beta | en |
dc.subject | cGAS-STING | en |
dc.subject | innate immune sensing | en |
dc.subject | C5a | en |
dc.subject | anaphylatoxin receptor | en |
dc.subject | C5aR2 | en |
dc.subject | pattern recognition receptor | en |
dc.title | C5aR2 Regulates STING-Mediated Interferon Beta Production in Human Macrophages | en |
dc.type | Journal Article | en |
dc.type.supercollection | scholarly_publications | en |
dc.type.supercollection | refereed_publications | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/laoneill | |
dc.identifier.rssinternalid | 266638 | |
dc.identifier.doi | http://dx.doi.org/10.3390/cells12232707 | |
dc.rights.ecaccessrights | openAccess | |
dc.identifier.rssuri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85179323181&doi=10.3390%2fcells12232707&partnerID=40&md5=d7d8e1ca74bcd17cab51e00c0a39af18 | |