dc.contributor.author | VINARDELL, TATIANA | en |
dc.contributor.author | KELLY, DANIEL | en |
dc.contributor.author | CAMPBELL, VERONICA ANN | en |
dc.contributor.author | BUCKLEY, CONOR TIMOTHY | en |
dc.date.accessioned | 2009-04-14T12:14:16Z | |
dc.date.available | 2009-04-14T12:14:16Z | |
dc.date.issued | 2008 | en |
dc.date.submitted | 2008 | en |
dc.identifier.citation | Thorpe, S. D., Buckley, C. T., Vinardell, T., O'Brien, F. J., Campbell, V. A., Kelly, D. J., Dynamic compression can inhibit chondrogenesis of mesenchymal stem cells., Biochemical and Biophysical Research Communications, 337, 2, 2008, 458-462 | en |
dc.identifier.other | Y | en |
dc.identifier.uri | http://hdl.handle.net/2262/29030 | |
dc.description | PUBLISHED | en |
dc.description | PMID: 18851955 | en |
dc.description.abstract | The objective of this study was to investigate the influence of dynamic compressive loading on chondrogenesis of mesenchymal stem cells (MSCs) in the presence of TGF-?3. Isolated porcine MSCs were suspended in 2% agarose and subjected to intermittent dynamic compression (10% strain) for a period of 42 days in a dynamic compression bioreactor. After 42 days in culture, the free-swelling specimens exhibited more intense alcian blue staining for proteoglycans, while immunohistochemical analysis revealed increased collagen type II immunoreactivity. Glycosaminoglycan (GAG) content increased with time for both free-swelling and dynamically loaded constructs, and by day 42 it was significantly higher in both the core (2.5 ? 0.21%w/w vs. 0.94 ? 0.03%w/w) and annulus (1.09 ? 0.09%w/w vs. 0.59 ? 0.08%w/w) of free-swelling constructs compared to dynamically loaded constructs. This result suggests that further optimization is required in controlling the biomechanical and/or the biochemical environment if such stimuli are to have beneficial effects in generating functional cartilaginous tissue. | en |
dc.description.sponsorship | Funding was provided by Science Foundation Ireland (07-RFP- ENMF142) and
Enterprise Ireland (PC / 2006/ 384). | en |
dc.format.extent | 458-462 | en |
dc.format.mimetype | application/pdf | |
dc.language.iso | en | en |
dc.relation.ispartofseries | Biochemical and Biophysical Research Communications | en |
dc.relation.ispartofseries | 337 | en |
dc.relation.ispartofseries | 2 | en |
dc.rights | Y | en |
dc.subject | Chondrogenesis | en |
dc.subject | Mesenchymal stem cells | en |
dc.subject | TGF-?3 | en |
dc.subject | Dynamic compression | en |
dc.subject | Tissue engineering | en |
dc.title | Dynamic compression can inhibit chondrogenesis of mesenchymal stem cells. | en |
dc.type | Journal Article | en |
dc.type.supercollection | scholarly_publications | en |
dc.type.supercollection | refereed_publications | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/kellyd9 | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/vinardt | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/vacmpbll | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/cbuckle | en |
dc.identifier.rssinternalid | 56667 | en |
dc.identifier.rssuri | http://dx.doi.org/10.1016/j.bbrc.2008.09.154 | |
dc.identifier.rssuri | http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WBK-4TN5GCK-B-7&_cdi=6713&_user=103681&_orig=search&_coverDate=12%2F12%2F2008&_sk=996229997&view=c&wchp=dGLbVzz-zSkzk&md5=9ebf2b016004cf9a1340007c7f7616dc&ie=/sdarticle.pdf | en |
dc.contributor.sponsor | Science Foundation Ireland | |
dc.contributor.sponsor | Enterprise Ireland | |