dc.contributor.author | MCGETTRICK-DILLON, ANNE | |
dc.contributor.author | O'NEILL, LUKE ANTHONY JOHN | |
dc.contributor.author | PALSSON, EVA | |
dc.date.accessioned | 2009-09-29T17:05:45Z | |
dc.date.available | 2009-09-29T17:05:45Z | |
dc.date.issued | 2006 | |
dc.date.submitted | 2006 | en |
dc.identifier.citation | McGettrick, A, Brint EK, Palsson-McDermott, EM, Rowe, DC, Golenbock, DT, Gay NJ, Fitzgerald, KA and O?Neill LA `Trif-related adapter molecule is phosphorylated by protein kinase C epsilon during Toll-like receptor 4 signalling? in Proceedings of the National Academy of Sciences, 103, (24), 2006, pp 9196 - 9201 | en |
dc.identifier.other | Y | en |
dc.identifier.other | Y | |
dc.identifier.uri | http://hdl.handle.net/2262/33442 | |
dc.description | PUBLISHED | en |
dc.description.abstract | PKC? has been shown to play a key role in the effect of the Gram-negative bacterial product LPS; however, the target for PKC? in LPS signaling is unknown. LPS signaling is mediated by Toll-like receptor 4, which uses four adapter proteins, MyD88, MyD88 adapter-like (Mal), Toll/IL-1R domain-containing adapter inducing IFN-? (Trif), and Trif-related adapter molecule (TRAM). Here we show that TRAM is transiently phosphorylated by PKC? on serine-16 in an LPS-dependent manner. Activation of IFN regulatory factor 3 and induction of the chemokine RANTES, which are both TRAM-dependent, were attenuated in PKC?-deficient cells. TRAMS16A is inactive when overexpressed and is attenuated in its ability to reconstitute signaling in TRAM-deficient cells. We have therefore uncovered a key process in Toll-like receptor 4 signaling, identifying TRAM as the target for PKC?. | en |
dc.description.sponsorship | We thank the Health Research Board and the Science Foundation of Ireland for funding this project | en |
dc.format.extent | 1016938 bytes | |
dc.format.extent | 9196 | en |
dc.format.extent | 9201 | en |
dc.format.mimetype | application/pdf | |
dc.language.iso | en | en |
dc.publisher | National Academy of Sciences | en |
dc.relation.ispartofseries | Proceedings of the National Academy of Sciences | en |
dc.relation.ispartofseries | 103 | en |
dc.relation.ispartofseries | 24 | en |
dc.rights | Y | en |
dc.subject | Biochemistry | en |
dc.title | Trif-related adapter molecule is phosphorylated by protein kinase C epsilon during Toll-like receptor 4 signalling | en |
dc.type | Journal Article | en |
dc.type.supercollection | scholarly_publications | en |
dc.type.supercollection | refereed_publications | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/laoneill | |
dc.identifier.rssinternalid | 45545 | |
dc.identifier.rssuri | http://dx.doi.org/10.1073/pnas.0600462103 | |
dc.contributor.sponsor | Health Research Board | |
dc.contributor.sponsor | Science Foundation Ireland | en |