dc.contributor.author | FARES, MARIO ALI | en |
dc.date.accessioned | 2010-02-01T14:57:10Z | |
dc.date.available | 2010-02-01T14:57:10Z | |
dc.date.issued | 2010 | en |
dc.date.submitted | 2010 | en |
dc.identifier.citation | Garceau V, Smith J, Paton IR, Davey M, Fares MA, Sester DP, Burt DW, Hume DA., Pivotal Advance: Avian colony-stimulating factor 1 (CSF-1), interleukin-34 (IL-34), and CSF-1 receptor genes and gene products, Journal of Leukocyte Biology, 87, 2010, 753-764 | en |
dc.identifier.other | Y | en |
dc.identifier.uri | http://hdl.handle.net/2262/36710 | |
dc.description | PUBLISHED | en |
dc.description.abstract | Macrophages are involved in many aspects of development, host defense, pathology, and homeostasis. Their normal differentiation, proliferation, and survival are controlled by CSF-1 via the activation of the CSF-1R. A recently discovered cytokine, IL-34, was shown to bind the same receptor in humans. Chicken is a widely used model organism in developmental biology, but the factors that control avian myelopoiesis have not been identified previously. The CSF-1, IL-34, and CSF1R genes in chicken and zebra finch were identified from respective genomic/cDNA sequence resources. Comparative analysis of the avian CSF1R loci revealed likely orthologs of mammalian macrophage-specific promoters and enhancers, and the CSF1R gene is expressed in the developing chick embryo in a pattern consistent with macrophage-specific expression. Chicken CSF-1 and IL-34 were expressed in HEK293 cells and shown to elicit macrophage growth from chicken BM cells in culture. Comparative sequence and co-evolution analysis across all vertebrates suggests that the two ligands interact with distinct regions of the CSF1R. These studies demonstrate that there are two separate ligands for a functional CSF1R across all vertebrates. | en |
dc.format.extent | 753-764 | en |
dc.format.mimetype | application/pdf | |
dc.language.iso | en | en |
dc.relation.ispartofseries | Journal of Leukocyte Biology | en |
dc.relation.ispartofseries | 87 | en |
dc.rights | Y | en |
dc.subject | Chicken | en |
dc.subject | zebra finch | en |
dc.subject | macrophage | en |
dc.subject | co-evolution | en |
dc.title | Pivotal Advance: Avian colony-stimulating factor 1 (CSF-1), interleukin-34 (IL-34), and CSF-1 receptor genes and gene products | en |
dc.type | Journal Article | en |
dc.type.supercollection | scholarly_publications | en |
dc.type.supercollection | refereed_publications | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/faresm | en |
dc.identifier.rssinternalid | 63301 | en |
dc.identifier.rssuri | http://dx.doi.org/10.1189/jlb.0909624 | en |
dc.identifier.rssuri | http://dx.doi.org/10.1189/jlb.0909624 | |