dc.contributor.author | KELLY, JOHN | en |
dc.contributor.author | MCCABE, THOMAS | en |
dc.date.accessioned | 2011-06-13T15:13:46Z | |
dc.date.available | 2011-06-13T15:13:46Z | |
dc.date.issued | 2010 | en |
dc.date.submitted | 2010 | en |
dc.identifier.citation | Vasudevan S, Smith JA, Wojdyla M, DiTrapani A, Kruger PE, McCabe T, Fletcher NC, Quinn SJ, Kelly JM, Substituted dipyridophenazine complexes of Cr(III): synthesis, enantiomeric resolution and binding interactions with calf thymus DNA., Dalton Transactions, 39, 16, 2010, 3990-8 | en |
dc.identifier.issn | 1477-9226 | en |
dc.identifier.other | Y | en |
dc.identifier.uri | http://hdl.handle.net/2262/56824 | |
dc.description | PUBLISHED | en |
dc.description.abstract | [Cr(phen)2(X2dppz)]3+ {X = H, Me, or F} have been synthesised, characterised, and chromatographically resolved into their constituent ? and ? enantiomers. The DNA-binding interactions of each of the racemic complexes were investigated, with the results of linear dichroism, thermal denaturation, and emission quenching studies indicative of intercalative binding to CT-DNA with a significant electrostatic contribution. UV/Vis absorption titrations suggest strong DNA binding by each of the racemic complexes, with the methylated analogue [Cr(phen)2(Me2dppz)]3+ exhibiting the largest equilibrium binding constant. Emission quenching and UV-Vis titrations of the enantiomers of [Cr(phen)2(dppz)]3+ imply similar binding affinities for the ? and ? isomers, although significant differences between the circular dichroism spectra of the enantiomers in the presence of DNA connote differences in binding orientation and/or conformation between the two. | en |
dc.description.sponsorship | SV and JAS thank IRCSET for the award of postdoctoral fellowships. The HEA (PRLTI4/CSCB) and SFI (06/CHP036 and 07/CHEF437) are thanked for their financial support. We are grateful to Dr Martin Feeney for recording the mass spectra. | en |
dc.format.extent | 3990-8 | en |
dc.language.iso | en | en |
dc.relation.ispartofseries | Dalton Transactions | en |
dc.relation.ispartofseries | 39 | en |
dc.relation.ispartofseries | 16 | en |
dc.rights | Y | en |
dc.subject | Genetics | en |
dc.subject | Cr(III) | en |
dc.title | Substituted dipyridophenazine complexes of Cr(III): synthesis, enantiomeric resolution and binding interactions with calf thymus DNA. | en |
dc.type | Journal Article | en |
dc.type.supercollection | scholarly_publications | en |
dc.type.supercollection | refereed_publications | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/tmccabe | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/jmkelly | en |
dc.identifier.rssinternalid | 72292 | en |
dc.identifier.doi | http://dx.doi.org/10.1039/c000150c | en |
dc.subject.TCDTheme | Genes & Society | en |
dc.identifier.rssuri | http://dx.doi.org/10.1039/C000150C | en |
dc.contributor.sponsor | Higher Education Authority (HEA) | en |
dc.contributor.sponsorGrantNumber | PRLTI4/CSCB | en |
dc.contributor.sponsor | Science Foundation Ireland (SFI) | en |
dc.contributor.sponsorGrantNumber | 07/CHEF437 | en |
dc.contributor.sponsor | Science Foundation Ireland (SFI) | en |
dc.contributor.sponsorGrantNumber | 06/CHP036 | en |
dc.contributor.sponsor | Irish Research Council for Science and Engineering Technology (IRCSET) | en |