dc.contributor.author | Finlay, David | en |
dc.date.accessioned | 2011-11-02T12:25:29Z | |
dc.date.available | 2011-11-02T12:25:29Z | |
dc.date.issued | 2010 | en |
dc.date.submitted | 2010 | en |
dc.identifier.citation | Finlay DK, Kelly AP, Clarke R, Sinclair LV, Deak M, Alessi DR, Cantrell DA, Temporal differences in the dependency on Phosphoinositide dependent kinase 1 distinguish the development of Vα14 iNKT cells, regulatory T cells and conventional T cells, Journal of Immunology, 185, 10, 2010, 5973 - 5982 | en |
dc.identifier.other | Y | en |
dc.identifier.uri | http://hdl.handle.net/2262/60447 | |
dc.description | PUBLISHED | en |
dc.description.abstract | The present study uses two independent genetic strategies to explore the requirement for Phosphoinositide dependent kinase-1 (PDK1) in the development of mature T cell populations from CD4/CD8 double positive thymocytes. The data show that CD4/CD8 double positive thymocytes that do not express PDK1 or express a catalytically inactive PDK1 mutant fail to produce mature invariant V?14 NKT cells but can differentiate to conventional CD4, CD8 or regulatory T cell subsets in the thymus. The PDK1 requirement for V?14 NKT cell development reflects that these cells require the PDK1 substrate Protein Kinase B (PKB, also called Akt) to meet the metabolic demands for proliferative expansion in response to Interleukin 15 or antigen receptor stimulation. There is also constitutive PDK1 signaling in conventional ?/? T cells that is not required for lineage commitment of these cells but fine-tunes the expression of coreceptors and adhesion molecules. Also, while PDK1 is dispensable for thymic development of conventional ?/? T cells, peripheral cells are substantially reduced. This reflects a PDK1 requirement for lymphopenia-induced proliferation, a process necessary for initial population of the peripheral T cell niche in neonatal mice. PDK1 is thus indispensable for T cell developmental programs but the timing of the PDK1 requirement is unique to different T cell subpopulations. | en |
dc.format.extent | 5973 | en |
dc.format.extent | 5982 | en |
dc.language.iso | en | en |
dc.relation.ispartofseries | Journal of Immunology | en |
dc.relation.ispartofseries | 185 | en |
dc.relation.ispartofseries | 10 | en |
dc.rights | Y | en |
dc.subject | Immunology | en |
dc.subject | T cells | en |
dc.title | Temporal differences in the dependency on Phosphoinositide dependent kinase 1 distinguish the development of Vα14 iNKT cells, regulatory T cells and conventional T cells | en |
dc.type | Journal Article | en |
dc.type.supercollection | scholarly_publications | en |
dc.type.supercollection | refereed_publications | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/finlayd | en |
dc.identifier.rssinternalid | 75182 | en |
dc.subject.TCDTheme | Immunology, Inflammation & Infection | en |
dc.identifier.rssuri | http://dx.crossref.org/10.4049%2Fjimmunol.1000827 | en |
dc.identifier.orcid_id | 0000-0003-2716-6679 | en |