dc.contributor.author | STREUBEL, GUNDULA | en |
dc.date.accessioned | 2014-12-11T10:42:14Z | |
dc.date.available | 2014-12-11T10:42:14Z | |
dc.date.issued | 2013 | en |
dc.date.submitted | 2013 | en |
dc.identifier.citation | Streubel,Gundula G., Bouchard,Caroline S. C.S., Berberich,Hannah H., Zeller,Marc S. M.S., Teichmann,Sophia S., Adamkiewicz,Jürgen J., Müller,Rolf Joachim R.J., Klempnauer,Karl Heinz K.H., Bauer,Uta Maria U.M., PRMT4 Is a Novel Coactivator of c-Myb-Dependent Transcription in Haematopoietic Cell Lines, PLoS Genetics, 9, 3, 2013 | en |
dc.identifier.issn | 15537390 | en |
dc.identifier.other | Y | en |
dc.identifier.uri | http://hdl.handle.net/2262/72418 | |
dc.description | PUBLISHED | en |
dc.description.abstract | Protein arginine methyltransferase 4 (PRMT4)–dependent methylation of arginine residues in histones and other chromatin-
associated proteins plays an important role in the regulation of gene expression. However, the exact mechanism of how
PRMT4 activates transcription remains elusive. Here, we identify the chromatin remodeller Mi2
a
as a novel interaction
partner of PRMT4. PRMT4 binds Mi2
a
and its close relative Mi2
b
, but not the other components of the repressive Mi2-
containing NuRD complex. In the search for the biological role of this interaction, we find that PRMT4 and Mi2
a
/
b
interact
with the transcription factor c-Myb and cooperatively coactivate c-Myb target gene expression in haematopoietic cell lines.
This coactivation requires the methyltransferase and ATPase activity of PRMT4 and Mi2, respectively. Chromatin
immunoprecipitation analysis shows that c-Myb target genes are direct transcriptional targets of PRMT4 and Mi2.
Knockdown of PRMT4 or Mi2
a
/
b
in haematopoietic cells of the erythroid lineage results in diminished transcriptional
induction of c-Myb target genes, attenuated cell growth and survival, and deregulated differentiation resembling the effects
caused by c-Myb depletion. These findings reveal an important and so far unknown connection between PRMT4 and the
chromatin remodeller Mi2 in c-Myb signalling. | en |
dc.description.sponsorship | This work was funded as part of the FOR531, TRR81, TRR17, and Ba 2292/1 by the DFG (Deutsche Forschungsgemeinschaft), as part of LOEWE (Tumor
and Inflammation) by the Land Hessen and by the BMBF (Bundesministerium fu
̈
r Bildung und Forschung) to U-MB. The funders had no role in study design, data
collection and analysis, decision to publish, or preparation of the manuscript | en |
dc.language.iso | en | en |
dc.relation.ispartofseries | PLoS Genetics | en |
dc.relation.ispartofseries | 9 | en |
dc.relation.ispartofseries | 3 | en |
dc.rights | Y | en |
dc.subject | c-Myb target genes | en |
dc.subject.lcsh | c-Myb target genes | en |
dc.title | PRMT4 Is a Novel Coactivator of c-Myb-Dependent Transcription in Haematopoietic Cell Lines | en |
dc.type | Journal Article | en |
dc.type.supercollection | scholarly_publications | en |
dc.type.supercollection | refereed_publications | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/streubeg | en |
dc.identifier.rssinternalid | 98319 | en |
dc.identifier.doi | http://dx.doi.org/10.1371/journal.pgen.1003343 | en |
dc.rights.ecaccessrights | openAccess | |