Controlled release of transforming growth factor-?3 from cartilage-extra-cellular-matrix-derived scaffolds to promote chondrogenesis of human-joint-tissue-derived stem cells.
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Journal ArticleDate:
2014Access:
openAccessCitation:
Almeida HV, Liu Y, Cunniffe GM, Mulhall KJ, Matsiko A, Buckley CT, O'Brien FJ, Kelly DJ, Controlled release of transforming growth factor-?3 from cartilage-extra-cellular-matrix-derived scaffolds to promote chondrogenesis of human-joint-tissue-derived stem cells., Acta biomaterialia, 10, 10, 2014, 4400-9Download Item:
Abstract:
Abstract: The objective of this study was to develop a scaffold derived from cartilaginous extracellular
matrix (ECM) that could be used as a growth factor delivery system to promote chondrogenesis of stem
cells. Dehyd
rothermal crosslinked scaffolds were fabricated using a slurry of homogenized porcine
articular cartilage, which were then seeded with human infrapatellar fat pad derived stem cells
(FPSCs). It was found that these ECM derived scaffolds promoted superior c
hondrogenesis of FPSCs
when the constructs were additionally stimulated with transforming growth factor (TGF)
-
β3. Cell
mediated contraction of the scaffold was observed, which could be limited by the additional use of 1
-
Ethyl
-
3
-
3dimethyl aminopropyl carbod
iimide (EDAC) crosslinking without suppressing cartilage
specific matrix accumulation within the construct. To further validate the utility of the ECM derived
scaffold, we next compared its chondro
-
permissive properties to a biomimetic collagen
-
hyaluronic
acid
(HA) scaffold optimized for cartilage tissue engineering (TE) applications. The cartilage ECM derived
scaffold supported at least comparable chondrogenesis to the collagen
-
HA scaffold, underwent less
contraction and retained a greater proportion of sy
nthesised sulphated glycosaminoglycans (sGAGs).
Having developed a promising scaffold for TE, with superior chondrogenesis observed in the presence
of exogenously supplied TGF
-
β3, the final phase of the study explored whether this scaffold could be
used as
a TGF
-
β3 delivery system to promote chondrogenesis of FPSCs. It was found that the majority
of TGF
-
β3 that was loaded onto the scaffold was released in a controlled manner over the first 10 days
of culture, with comparable long
-
term chondrogenesis observe
d in these TGF
-
β3 loaded constructs
compared to scaffolds where the TGF
-
β3 was continuously added to the media. The results of this
study support the use of cartilage ECM derived scaffolds as a growth factor delivery system for use in
articular cartilage r
egeneration
Author's Homepage:
http://people.tcd.ie/kellyd9http://people.tcd.ie/cbuckle
http://people.tcd.ie/cunnifg
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PUBLISHEDType of material:
Journal ArticleSeries/Report no:
Acta biomaterialia10
10
Availability:
Full text availableSubject (TCD):
Next Generation Medical DevicesDOI:
http://dx.doi.org/10.1016/j.actbio.2014.05.030ISSN:
1742-7061Metadata
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