dc.contributor.author | KELLY, DANIEL | en |
dc.contributor.author | VINARDELL, TATIANA | en |
dc.contributor.author | CUNNIFFE, GR?INNE | en |
dc.contributor.author | O'BRIEN, FERGAL | en |
dc.date.accessioned | 2015-12-09T11:48:36Z | |
dc.date.available | 2015-12-09T11:48:36Z | |
dc.date.issued | 2015 | en |
dc.date.submitted | 2015 | en |
dc.identifier.citation | Cunniffe, G.M., Vinardell, T., Murphy, J.M., (...), O'Brien, F.J., Kelly, D.J., Porous decellularized tissue engineered hypertrophic cartilage as a scaffold for large bone defect healing, Acta Biomaterialia, 23, 2015, 82?90 | en |
dc.identifier.other | Y | en |
dc.identifier.uri | http://hdl.handle.net/2262/75178 | |
dc.description | PUBLISHED | en |
dc.description.abstract | Clinical translation of tissue engineered therapeutics is hampered by the significant logistical and regulatory challenges associated with such products, prompting increased interest in the use of decellularized extracellular matrix (ECM) to enhance endogenous regeneration. Most bones develop and heal by endochondral ossification, the replacement of a hypertrophic cartilaginous intermediary with bone. The hypothesis of this study is that a porous scaffold derived from decellularized tissue engineered hypertrophic cartilage will retain the necessary signals to instruct host cells to accelerate endogenous bone regeneration. Cartilage tissue (CT) and hypertrophic cartilage tissue (HT) were engineered using human bone marrow derived mesenchymal stem cells, decellularized and the remaining ECM was freeze-dried to generate porous scaffolds. When implanted subcutaneously in nude mice, only the decellularized HT-derived scaffolds were found to induce vascularization and de novo mineral accumulation. Furthermore, when implanted into critically-sized femoral defects, full bridging was observed in half of the defects treated with HT scaffolds, while no evidence of such bridging was found in empty controls. Host cells which had migrated throughout the scaffold were capable of producing new bone tissue, in contrast to fibrous tissue formation within empty controls. These results demonstrate the capacity of decellularized engineered tissues as ‘off-the-shelf’ implants to promote tissue regeneration | en |
dc.description.sponsorship | This work was funded by the AO Foundation under the large bone defect healing program. Surgical advice, PEEK fixator plates and custom designed surgical instruments were kindly provided by Professor Steve Goldstein, University of Michigan. Human bone marrow-derived MSCs were kindly provided by REMEDI, National Centre for Biomedical Engineering Science, National University of Ireland Galway, Ireland and funded by Science Foundation Ireland (Grant Number 09/SRC/B1794). This work was also funded by the Health Research Board of Ireland (HRA_POR/2011/27) and the European Research Council (ERC grant agreement no. 239685) | en |
dc.format.extent | 82?90 | en |
dc.relation.ispartofseries | Acta Biomaterialia | en |
dc.relation.ispartofseries | 23 | en |
dc.rights | Y | en |
dc.subject | decellularized extracellular matrix (ECM) | en |
dc.subject.lcsh | decellularized extracellular matrix (ECM) | en |
dc.title | Porous decellularized tissue engineered hypertrophic cartilage as a scaffold for large bone defect healing | en |
dc.type | Journal Article | en |
dc.type.supercollection | scholarly_publications | en |
dc.type.supercollection | refereed_publications | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/kellyd9 | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/vinardt | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/fobrien | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/cunnifg | en |
dc.identifier.rssinternalid | 104665 | en |
dc.identifier.doi | http://dx.doi.org/10.1016/j.actbio.2015.05.031 | en |
dc.rights.ecaccessrights | openAccess | |
dc.identifier.orcid_id | 0000-0003-4091-0992 | en |
dc.contributor.sponsor | Science Foundation Ireland (SFI) | en |
dc.contributor.sponsorGrantNumber | 09/SRC/B1794 | en |