Indentification and functionality of antigens reacting with antisperm antibodies and their role in male immunoinfertility
Citation:
William N. Jagoe, 'Indentification and functionality of antigens reacting with antisperm antibodies and their role in male immunoinfertility', [thesis], Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2014, pp 339Download Item:
Abstract:
Antisperm antibodies (ASA) are a leading cause of male associated infertility. The male partner of 1 in 10 couples tests positive for sperm bound antibodies with immunoglobulins of the IgG class being found in all patients and IgA to a lower extent. This study used several approaches to identify novel sperm surface antigens. Firstly, reactivity to mouse reproductive tract antigens were invesigated, indentitying CRISP 1 and MVDP as targets for human ASA. Both proteins were purified to homogeneity. CRISP 1 was shown to be involved in sperm-egg binding, as polyclonal anti-CRISP 1 antibodies blocked mouse ova fertilization in vitro. MVDP, a member of the aldo-keto reductase family, was revealed to function in the detoxification of acrolein, a reactive aldehyde produced by the catabolism of the polyamines, spermine and spermidine, which are abundant in seminal plasma. Secondly, human seminal plasma proteins were explored as targets for ASA.
Author: Jagoe, William N.
Advisor:
Carroll, JoeKelly, Vincent
Qualification name:
Doctor of Philosophy (Ph.D.)Publisher:
Trinity College (Dublin, Ireland). School of Biochemistry and ImmunologyNote:
TARA (Trinity’s Access to Research Archive) has a robust takedown policy. Please contact us if you have any concerns: rssadmin@tcd.ieType of material:
thesisAvailability:
Full text availableMetadata
Show full item recordLicences: