dc.contributor.author | Senge, Mathias | en |
dc.date.accessioned | 2019-12-02T12:11:21Z | |
dc.date.available | 2019-12-02T12:11:21Z | |
dc.date.created | 2018 | en |
dc.date.issued | 2018 | en |
dc.date.submitted | 2018 | en |
dc.identifier | 29596749 | en |
dc.identifier.citation | Belali Simin, Savoie Huguette, O'Brien Jessica M, Cafolla Atillio A, O'Connell Barry, Karimi Ali Reza, Boyle Ross W, Senge Mathias O, Synthesis and Characterization of Temperature-Sensitive and Chemically Cross-Linked Poly( N-isopropylacrylamide)/Photosensitizer Hydrogels for Applications in Photodynamic Therapy, Biomacromolecules, 19, 5, 2018, 1592 - 1601 | en |
dc.identifier.issn | 1525-7797 | en |
dc.identifier.other | Y | en |
dc.identifier.uri | https://pubs.acs.org/doi/10.1021/acs.biomac.8b00293 | |
dc.identifier.uri | http://hdl.handle.net/2262/90935 | |
dc.description | PUBLISHED | en |
dc.description.abstract | A novel poly(N-isopropylacrylamide) (PNIPAM) hydrogel containing different photosensitizers (protoporphyrin IX (PpIX), pheophorbide a (Pba), and protoporphyrin IX dimethyl ester (PpIX-DME)) has been synthesized with a significant improvement in water solubility and potential for PDT applications compared to the individual photosensitizers (PSs). Conjugation of PpIX, Pba, and PpIX-DME to the poly(N-isopropylacrylamide) chain was achieved using the dispersion polymerization method. This study describes how the use of nanohydrogel structures to deliver a photosensitizer with low water solubility and high aggregation tendencies in polar solvents overcomes these limitations. FT-IR spectroscopy, UV–vis spectroscopy, 1H NMR, fluorescence spectroscopy, SEM, and DLS analysis were used to characterize the PNIPAM–photosensitizer nanohydrogels. Spectroscopic studies indicate that the PpIX, Pba, and PpIX-DME photosensitizers are covalently conjugated to the polymer chains, which prevents aggregation and thus allows significant singlet oxygen production upon illumination. Likewise, the lower critical solution temperature was raised to ∼44 °C in the new PNIPAM-PS hydrogels. The PNIPAM hydrogels are biocompatible with >90% cell viability even at high concentrations of the photosensitizer in vitro. Furthermore, a very sharp onset of light-dependent toxicity for the PpIX-based nanohydrogel in the nanomolar range and a more modest, but significant, photocytotoxic response for Pba-PNIPAM and PpIX-DME-PNIPAM nanohydrogels suggest that the new hydrogels have potential for applications in photodynamic therapy. | en |
dc.format.extent | 1592 | en |
dc.format.extent | 1601 | en |
dc.language.iso | en | en |
dc.relation.ispartofseries | Biomacromolecules | en |
dc.relation.ispartofseries | 19 | en |
dc.relation.ispartofseries | 5 | en |
dc.rights | Y | en |
dc.subject | Protoporphyrin IX | en |
dc.subject | Hydrogels | en |
dc.subject | Photodynamic therapy | en |
dc.subject | Poly(N-isopropylacrylamide) | en |
dc.subject | Photosensitizer | en |
dc.subject | Pheophorbide a | en |
dc.title | Synthesis and Characterization of Temperature-Sensitive and Chemically Cross-Linked Poly( N-isopropylacrylamide)/Photosensitizer Hydrogels for Applications in Photodynamic Therapy | en |
dc.title.alternative | Biomacromolecules | en |
dc.type | Journal Article | en |
dc.type.supercollection | scholarly_publications | en |
dc.type.supercollection | refereed_publications | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/sengem | en |
dc.identifier.rssinternalid | 195778 | en |
dc.identifier.doi | http://dx.doi.org/10.1021/acs.biomac.8b00293 | en |
dc.rights.ecaccessrights | openAccess | |
dc.identifier.rssuri | http://europepmc.org/abstract/MED/29596749 https://doi.org/10.1021/acs.biomac.8b00293 | en |
dc.contributor.sponsor | Science Foundation Ireland (SFI) | en |
dc.contributor.sponsorGrantNumber | IvP 13/IA/1894 | en |