dc.contributor.author | O'DRISCOLL, LORRAINE | en |
dc.date.accessioned | 2010-04-30T14:40:31Z | |
dc.date.available | 2010-04-30T14:40:31Z | |
dc.date.issued | 2010 | en |
dc.date.submitted | 2010 | en |
dc.identifier.citation | Hennessy E, Clynes M, Jeppesen P, O'Driscoll L, Identification of microRNAs with a role in glucose stimulated insulin secretion by expression profiling of MIN6 cells., Biochemical and Biophysical Research Communications, 396, 2, 2010, 457 - 462 | en |
dc.identifier.other | Y | en |
dc.identifier.uri | http://hdl.handle.net/2262/39365 | |
dc.description | PUBLISHED | en |
dc.description.abstract | MicroRNAs (miRNAs) are a family of endogenous small non-coding RNAs which regulate mRNAs at the post-transcriptional level. MiRNAs have been identified in both normal physiological and pathological conditions. To date, a limited number of miRNAs have been shown to be involved in the regulation of insulin secretion. We have identified a panel of 10 miRNAs down-regulated in glucose non-responsive MIN6 cells compared to glucose responsive cells using TaqMan Low Density human microRNA arrays. Of these 10 miRNA targets, subsequent functional investigations involving knockdown of mir-200a, mir-130a and mir-410 levels suggested that they may decrease the capability of MIN6 cells to secrete insulin in response to stimulatory levels of glucose. Conversely, experiment with over-expression of mir-410 suggest that it may enhance levels of glucose stimulated insulin secretion. In this study, we have also identified 21 miRNAs not previously known to have a potential murine homolog. | en |
dc.format.extent | 457 | en |
dc.format.extent | 462 | en |
dc.language.iso | en | en |
dc.relation.ispartofseries | Biochemical and Biophysical Research Communications | en |
dc.relation.ispartofseries | 396 | en |
dc.relation.ispartofseries | 2 | en |
dc.rights | Y | en |
dc.subject | microRNA | en |
dc.subject | GSIS | en |
dc.subject | insulin secretion | en |
dc.subject | MIN6 | en |
dc.title | Identification of microRNAs with a role in glucose stimulated insulin secretion by expression profiling of MIN6 cells. | en |
dc.type | Journal Article | en |
dc.type.supercollection | scholarly_publications | en |
dc.type.supercollection | refereed_publications | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/lodrisc | en |
dc.identifier.rssinternalid | 66149 | en |
dc.identifier.doi | http://dx.doi.org/10.1016/j.bbrc.2010.04.116 | en |
dc.subject.TCDTheme | Genes & Society | en |
dc.identifier.rssuri | http://hdl.handle.net/2262/39365 | en |
dc.identifier.orcid_id | 0000-0002-9860-8262 | en |
dc.contributor.sponsor | Health Research Board (HRB) | en |