Browsing by Author "Southern, John"
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De-novo designed ??-lysine derivatives can both augment and diminish the proliferation rates of E. coli through the action of Elongation Factor P
Kelly, Vincent; Southern, John; Connon, Stephen (2022)An investigation into the effect of modified β-lysines on the growth rates of eubacterial cells is reported. It is shown that the effects observed are due to the post translational modification of Elongation Factor P (EFP) ... -
The eukaryotic tRNA-guanine transglycosylase enzyme inserts queuine into tRNA via a sequential bi?bi mechanism
Southern, John; Kelly, Vincent; Connon, Stephen (2020)Eukaryotic tRNA-guanine transglycosylase (TGT) – an enzyme recently recognised to be of potential therapeutic importance – catalyses base-exchange of guanine for queuine at the wobble position of tRNAs associated with ... -
The human tRNA-guanine transglycosylase displays promiscuous nucleobase preference but strict tRNA specificity.
Kelly, Vincent; Senge, Mathias; Southern, John; Hokamp, Karsten; Connon, Stephen (2021)Base-modification can occur throughout a transfer RNA molecule; however, elaboration is particularly prevalent at position 34 of the anticodon loop (the wobble position), where it functions to influence protein translation. ...