dc.contributor.author | Dunne, Margaret | |
dc.contributor.author | MANGAN, BOZGANA | |
dc.contributor.author | DOHERTY, DEREK | |
dc.date.accessioned | 2011-04-15T14:58:22Z | |
dc.date.available | 2011-04-15T14:58:22Z | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010 | en |
dc.identifier.citation | Dunne MR, Mangan BA, Madrigal-Estebas L, Doherty DG, Preferential Th1 Cytokine Profile of Phosphoantigen-Stimulated Human V?9V?2 T Cells, Mediators of Inflammation, 2010, 704941, 2010 | en |
dc.identifier.other | Y | |
dc.identifier.uri | http://hdl.handle.net/2262/54842 | |
dc.description | PUBLISHED | en |
dc.description.abstract | Human V?9V?2 T cells recognise pyrophosphate-based antigens (phosphoantigens) and have multiple functions in innate and adaptive immunity, including a unique ability to activate other cells of the immune system. We used flow cytometry and ELISA to define the early cytokine profiles of V?9V?2 T cells stimulated in vitro with isopentenyl pyrophosphate (IPP) and (E)-4-hydroxy-3-methyl-but-2 enyl pyrophosphate (HMB-PP) in the absence and presence of IL-2 and IL-15. We show that fresh V?9V?2 T cells produce interferon-? (IFN-?) and tumour necrosis factor-? (TNF-?) within 4 hours of stimulation with phosphoantigen, but neither IL-10, IL-13, nor IL-17 was detectable up to 72 hours under these conditions. Cytokine production was not influenced by expression or lack, thereof, of CD4 or CD8. Addition of IL-2 or IL-15 caused expansion of IFN-?-producing V?9V?2 T cells, but did not enhance IFN-? secretion after 24-72 hours. Thus, phosphoantigen-stimulated V?9V?2 T cells have potential as Th1-biasing adjuvants for immunotherapy. | en |
dc.description.sponsorship | This work was supported by grants from the Irish Research Council for Science Engineering and Technology, the Health Research Board, and Science Foundation Ireland. | en |
dc.language.iso | en | en |
dc.publisher | Hindawi | en |
dc.relation.ispartofseries | Mediators of Inflammation; | |
dc.relation.ispartofseries | 2010; | |
dc.relation.ispartofseries | 704941; | |
dc.rights | Y | en |
dc.subject | Immunology | en |
dc.subject | T Cells | en |
dc.title | Preferential Th1 Cytokine Profile of Phosphoantigen-Stimulated Human V?9V?2 T Cells | en |
dc.type | Journal Article | en |
dc.type.supercollection | scholarly_publications | en |
dc.type.supercollection | refereed_publications | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/dunnem12 | |
dc.identifier.peoplefinderurl | http://people.tcd.ie/dohertde | |
dc.identifier.rssinternalid | 72729 | |
dc.identifier.rssuri | http://dx.doi.org/10.1155/2010/704941 | en |
dc.contributor.sponsor | Health Research Board | en |
dc.contributor.sponsor | Science Foundation Ireland | en |
dc.contributor.sponsor | Irish Research Council for Science Engineering and Technology | en |