dc.contributor.author | O'DONNELL, JAMES | |
dc.date.accessioned | 2011-10-05T14:20:52Z | |
dc.date.available | 2011-10-05T14:20:52Z | |
dc.date.issued | 2011 | |
dc.date.submitted | 2011 | en |
dc.identifier.citation | Vanessa Castro-Lopez, Leanne F. Harris, James S. O'Donnell, Anthony J. Killard, Quantification of unfractionated heparin in human plasma and whole blood by means of novel fluorogenic anti-FXa assays, Journal of Pharmaceutical and Biomedical Analysis, 56, 5, 2011, 992-997 | en |
dc.identifier.other | Y | |
dc.identifier.uri | http://hdl.handle.net/2262/59846 | |
dc.description | PUBLISHED | en |
dc.description.abstract | Novel and sensitive plate-based fluorogenic anti-factor Xa (FXa) assays were investigated to quantify unfractionated heparin (UFH) in human plasma and whole blood within the therapeutic ranges of 0-1.6 U/mL and 0?0.8 U/mL, respectively. Two fluorogenic anti-FXa assay methods were defined for low (0-0.6 U/mL) and high (0.6-1.2 U/mL) pharmacologically relevant UFH concentration ranges in pooled human plasma. In both cases significant differences were observed at intervals of 0.2 U/mL (P < 0.05). The semi-logarithmic plots of the calibration curves in the low and high UFH range were both fitted to linear regressions with correlation coefficients of 0.96 and >0.99, respectively. The assay was also optimized for whole blood which was capable of differentiating UFH concentrations at intervals of 0.2 U/mL (P < 0.05) in the range of 0-0.4 U/mL. The statistically different results were fitted to a linear regression with a correlation coefficient of >0.99. The results obtained in this study could assist diagnostic laboratories towards improved monitoring of UFH therapy. | en |
dc.format.extent | 992-997 | en |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.relation.ispartofseries | Journal of Pharmaceutical and Biomedical Analysis; | |
dc.relation.ispartofseries | 56; | |
dc.relation.ispartofseries | 5; | |
dc.rights | Y | en |
dc.subject | Hematology | en |
dc.subject | human plasma | en |
dc.title | Quantification of unfractionated heparin in human plasma and whole blood by means of novel fluorogenic anti-FXa assays | en |
dc.type | Journal Article | en |
dc.type.supercollection | scholarly_publications | en |
dc.type.supercollection | refereed_publications | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/jodonne | |
dc.identifier.rssinternalid | 74442 | |
dc.identifier.rssuri | http://dx.doi.org/10.1016/j.jpba.2011.07.025 | en |
dc.contributor.sponsor | Enterprise Ireland | en |
dc.contributor.sponsorGrantNumber | TD/2009/0124 | en |