dc.contributor.advisor | Spiers, Paul | |
dc.contributor.author | Rietz, Anne | |
dc.date.accessioned | 2017-02-08T14:37:03Z | |
dc.date.available | 2017-02-08T14:37:03Z | |
dc.date.issued | 2011 | |
dc.identifier.citation | Anne Rietz, 'β-adrenoceptor-mediated regulation of matrix metalloproteinase-9 in fibroblasts', [thesis], Trinity College (Dublin, Ireland). School of Medicine. Discipline of Pharmacology & Therapeutics, 2011, pp 256 | |
dc.identifier.other | THESIS 9260 | |
dc.identifier.uri | http://hdl.handle.net/2262/79331 | |
dc.description.abstract | Adrenoceptor signalling in fibroblasts: Intracellular cAMP levels were measured by a direct cAMP enzyme immunoassay. Isoprenaline (ISO, 1 μM) increased intracellular cAMP concentration by 1.3 and 4.8 fold, P<0.05, after 10 min in the NIH3T3 fibroblasts and human cardiac ventricular fibroblasts (HCVF) respectively. These effects were abolished by pre-treatment with propranolol (PROP, 10 μM; P<0.05). Nuclear translocation of phospho-CREB was increased by isoprenaline (0.1 μM) and phenylephrine (10 μM) increased in NIH3T3 fibroblasts (P<0.05). Western blot analysis indicated that isoprenaline (1 μM) and phenylephrine (10 μM) caused a biphasic increase in p38 MAPK and a sustained increase in ERK1/2 MAPK activation over a 30 min period in NIH3T3 fibroblasts. However, in HCVF cells, isoprenaline (1 μM) decreased ERKl/2 MAPK phosphorylation by 70% after 30 min, an effect abolished by propranolol (10 μM; ISO vs. ISO/PROP: 0.30±0.06 vs. 1.05±0.10 AU; P<0.05). | |
dc.format | 1 volume | |
dc.language.iso | en | |
dc.publisher | Trinity College (Dublin, Ireland). School of Medicine. Discipline of Pharmacology & Therapeutics | |
dc.relation.isversionof | http://stella.catalogue.tcd.ie/iii/encore/record/C__Rb14726656 | |
dc.subject | Pharmacology and Therapeutics, Ph.D. | |
dc.subject | Ph.D. Trinity College Dublin | |
dc.title | β-adrenoceptor-mediated regulation of matrix metalloproteinase-9 in fibroblasts | |
dc.type | thesis | |
dc.type.supercollection | thesis_dissertations | |
dc.type.supercollection | refereed_publications | |
dc.type.qualificationlevel | Doctoral | |
dc.type.qualificationname | Doctor of Philosophy (Ph.D.) | |
dc.rights.ecaccessrights | openAccess | |
dc.format.extentpagination | pp 256 | |
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