dc.contributor.author | Dev, Kumlesh | en |
dc.date.accessioned | 2019-08-21T13:08:57Z | |
dc.date.available | 2019-08-21T13:08:57Z | |
dc.date.issued | 2002 | en |
dc.date.submitted | 2002 | en |
dc.identifier.citation | H. Hirbec, O. Perestenko, A. Nishimune, G. Meyer, S. Nakanishi, J.M. Henley, and K.K. Dev, The PDZ Proteins PICK1, GRIP and Syntenin bind multiple glutamate receptor subtypes, J. Biol Chem, 277, 2002, 15221 - 15224 | en |
dc.identifier.other | Y | en |
dc.identifier.uri | http://hdl.handle.net/2262/89271 | |
dc.description | PUBLISHED | en |
dc.description.abstract | Using sequence homology searches, yeast two-hybrid assays and glutathione S-transferase (GST)-pull-down approaches we have identified a series of glutamate receptor subunits that interact differentially with the PDZ proteins GRIP, PICK1, and syntenin. GST-pull-down experiments identified more interactions than detected by yeast two-hybrid assays. We report several receptor-protein interactions, strong ones include: (i) GRIP and syntenin with mGluR7a, mGluR4a, and mGluR6; (ii) PICK1 and GRIP with mGluR3; and (iii) syntenin with all forms of GluR1–4 and mGluR7b. We further characterized the novel mGluR7a-GRIP interaction found both in yeast two-hybrid and GST-pull-down assays and observed that mGluR7a localization overlapped with GRIP with in hippocampal neurons. The wide range of targets for PICK1, GRIP, and syntenin suggests they may represent a molecular mechanism that can concentrate and/or regulate a number of different receptors at a common site on a synapse. These data also suggest that the structural determinants involved in PDZ interactions are more complex than originally envisaged. | en |
dc.format.extent | 15221 | en |
dc.format.extent | 15224 | en |
dc.language.iso | en | en |
dc.relation.ispartofseries | J. Biol Chem | en |
dc.relation.ispartofseries | 277 | en |
dc.rights | Y | en |
dc.subject | (GST)-pull-down approach | en |
dc.subject | Sequence homology searches | en |
dc.subject | Yeast two-hybrid assays | en |
dc.subject | Glutamate receptor subunits | en |
dc.subject | PDZ proteins | en |
dc.subject | GRIP | en |
dc.subject | PICK1 | en |
dc.subject | Syntenin | en |
dc.title | The PDZ Proteins PICK1, GRIP and Syntenin bind multiple glutamate receptor subtypes | en |
dc.type | Journal Article | en |
dc.type.supercollection | scholarly_publications | en |
dc.type.supercollection | refereed_publications | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/devk | en |
dc.identifier.rssinternalid | 57126 | en |
dc.rights.ecaccessrights | openAccess | |
dc.subject.TCDTheme | Neuroscience | en |
dc.identifier.orcid_id | 0000-0003-4274-9119 | en |