Simultaneous disruption of Interleukin (IL)-4 and IL-13 defines individual roles in T Helper cell Type 2-mediated responses
Citation:
McKenzie, G.J., Fallon, P.G., Emson, C.L., Grencis, R.K. & McKenzie, A.N.J., Simultaneous disruption of Interleukin (IL)-4 and IL-13 defines individual roles in T Helper cell Type 2-mediated responses, Journal of Experimental Medicine, 189, 1999, 1565 - 1572Download Item:
Abstract:
Using a single vector targeting strategy, we have generated mice with a combined deficiency of
interleukin (IL)-4 and IL-13 to clarify their roles in T helper type 2 (Th2) cell responses. Using
immunological challenges normally characterized by a Th2-like response, we have compared
the responses of the double-deficient mice with those generated by wild-type, IL-4?deficient,
and IL-13?deficient mice. Using a pulmonary granuloma model, induced with Schistosoma
mansoni eggs, we demonstrate that although eosinophil infiltration, immunoglobulin E, and IL-5
production are reduced in the IL-4?deficient mice and IL-13?deficient mice, they are abolished
only in the combined absence of both cytokines. Furthermore, IL-4/13?deficient animals
are severely impaired in their ability to expel the gastrointestinal nematode Nippostrongylus brasiliensis.
Unexpectedly, N. brasiliensis?infected IL-4/13?deficient mice developed elevated IL-5
and eosinophilia, indicating that compensatory mechanisms exist for the expression of IL-5, although
serum IgE remained undetectable. IL-4/13?deficient mice default to a Th1-like phenotype
characterized by the expression of interferon
g and the production of IgG2a and IgG2b.
We conclude that IL-4 and IL-13 cooperate to initiate rapid Th2 cell?driven responses, and
that although their functions overlap, they perform additive roles.
Author's Homepage:
http://people.tcd.ie/pfallonDescription:
PUBLISHED
Author: FALLON, PADRAIC GERARD
Publisher:
The Rockefeller University PressType of material:
Journal ArticleCollections
Series/Report no:
Journal of Experimental Medicine;189;
Availability:
Full text availableKeywords:
Immunology, IL-4/13, IL-4/14Metadata
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