dc.contributor.author | FREELEY, MICHAEL | en |
dc.date.accessioned | 2015-01-23T11:58:27Z | |
dc.date.available | 2015-01-23T11:58:27Z | |
dc.date.issued | 2013 | en |
dc.date.submitted | 2013 | en |
dc.identifier.citation | Byrne G, Freeley M, Feighery C, Whelan A and Long A, Protein kinase C delta is a substrate of tissue transglutaminase and a novel autoantigen in coeliac disease, Clinical Immunology, 147, 1, 2013, 1 - 8 | en |
dc.identifier.other | Y | en |
dc.identifier.uri | http://hdl.handle.net/2262/73116 | |
dc.description | PUBLISHED | en |
dc.description.abstract | Post-translational modification of proteins by deamidation or transamidation by tissue transglutaminase (tTG) has been suggested as a possible mechanism for the development of autoimmunity. Sequence analysis of protein kinase C delta (PKCδ) identified an amino acid motif that suggested the possibility that PKCδ was a glutamine substrate of tTG and MALDI-TOF analysis of synthesised peptides from PKCδ proved that this was the case. Polymerisation experiments using recombinant tTG and biotinylated hexapeptide substrate incorporation assays demonstrated that PKCδ is a substrate for tTG-mediated transamidation. Elevated levels of anti-PKCδ antibodies were detected in sera from patients with coeliac disease (p<0.0001) but not from patients with other autoimmune disorders. These data suggest that a subset of patients with coeliac disease produce autoantibodies against PKCδ and that this response may stem from a tTG-PKCδ substrate interaction. | en |
dc.format.extent | 1 | en |
dc.format.extent | 8 | en |
dc.language.iso | en | en |
dc.relation.ispartofseries | Clinical Immunology | en |
dc.relation.ispartofseries | 147 | en |
dc.relation.ispartofseries | 1 | en |
dc.rights | Y | en |
dc.subject | coeliac disease | en |
dc.title | Protein kinase C delta is a substrate of tissue transglutaminase and a novel autoantigen in coeliac disease | en |
dc.type | Journal Article | en |
dc.type.supercollection | scholarly_publications | en |
dc.type.supercollection | refereed_publications | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/freeleym | en |
dc.identifier.rssinternalid | 83806 | en |
dc.rights.ecaccessrights | openAccess | |