Post-transcriptional Dysregulation by microRNAs is implicated in the Pathogenesis of Gastrointestinal Stromal Tumour [GIST]

Abstract

In a cohort of 73 GISTs including adult mutant, adult wild-type and paediatric cases miRNA expression was examined using TaqMan® Low Density Arrays (TLDAs), allowing the profiling of 667 miRNAs in a set of two (pool A and B) 384well cards. Individual TaqMan® miRNA assays used to validate the arrays cards showed good concordance with array results. Unsupervised hierarchical clustering splits the cases into two large clusters A and B, which are further subdivided into clusters B1 and B2. Adult mutant cases are located in clusters A and B1 and paediatric cases in cluster B2.